Open AccessThe product of the lexC gene of Escherichia coli is single-stranded DNA-binding protein
Author(s) -
Ralph R. Meyer,
Diane C. Rein,
Jeffrey Glassberg
Publication year - 1982
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.150.1.433-435.1982
Subject(s) - biology , single stranded binding protein , dna , escherichia coli , dna binding protein , microbiology and biotechnology , dna replication , gene , binding protein , gene product , ddb1 , seqa protein domain , biochemistry , origin of replication , gene expression , transcription factor
Extracts from lexC113 cells could not support phage G4 DNA-dependent replication unless supplemented with single-stranded DNA-binding protein. Purified lexC113 binding protein supported synthesis in a reconstituted replication assay, using purified proteins at 30 but not at 42 degrees C, indicating that the product of the lexC113 gene is an altered single-stranded DNA-binding protein.
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