Isolation and characterization of Pseudomonas aeruginosa R' plasmids constructed by interspecific mating | Zendy

A F Morgan | Zendy

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Isolation and characterization of Pseudomonas aeruginosa R' plasmids constructed by interspecific mating

Author(s) -

A F Morgan

Publication year - 1982

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.149.2.654-661.1982

Subject(s) - biology , plasmid , pseudomonas aeruginosa , auxotrophy , genetics , locus (genetics) , interspecific competition , pseudomonas putida , recombination , isolation (microbiology) , microbiology and biotechnology , escherichia coli , gene , bacteria , botany

Plasmid R68.45 was used to construct R' plasmids carrying a maximum of 4 to 5 map minutes of the Pseudomonas aeruginosa PAO chromosome by interspecific mating, using P. putida PPN as the recipient. These R' plasmids were used to determine the map location of the amiE locus and to identify tentatively a number of P. putida auxotrophic mutations. Some of these R' plasmids could not be maintained in recombination-deficient P. aeruginosa strains.

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