P1 transduction mapping of the trg locus in rac+ and rac strains of Escherichia coli K-12. | Zendy

Rex Bitner | Zendy; Peter L. Kuempel | Zendy

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P1 transduction mapping of the trg locus in rac+ and rac strains of Escherichia coli K-12.

Author(s) -

Rex Bitner,

Peter L. Kuempel

Publication year - 1982

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.149.2.529-533.1982

Subject(s) - biology , escherichia coli , locus (genetics) , transduction (biophysics) , genetics , signal transduction , microbiology and biotechnology , gene , biochemistry

The trg locus, which had been located at min 31 in the cotransduction gap in the terminus region of the chromosome of Escherichia coli, has been mapped by transduction with bacteriophage P1. This locus exhibited no cotransduction with fnr when rac+ strains were used. If rac strains were used, which removed approximately 27 kilobase pairs of DNA, trg and fnr exhibited 8.2% cotransduction. Although this mapping of trg at min 31.1 considerably reduces the size of the cotransduction gap, trg exhibited no cotransduction with a Tn10 insertion located on the other side of the gap at min 34.2.

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