ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt | Zendy

Jennifer A. Thomson | Zendy; P D Gerstenberger | Zendy; Daniel E. Goldberg | Zendy; E Gociar | Zendy; A Orozco de Silva | Zendy; D G Fraenkel | Zendy

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ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt

Author(s) -

Jennifer A. Thomson,

P D Gerstenberger,

Daniel E. Goldberg,

E Gociar,

A Orozco de Silva,

D G Fraenkel

Publication year - 1979

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.137.1.502-506.1979

Subject(s) - biology , glucose 6 phosphate isomerase , aldolase a , biochemistry , plasmid , escherichia coli , complementation , phosphoglycerate kinase , phosphofructokinase , pentose phosphate pathway , microbiology and biotechnology , isomerase , triosephosphate isomerase , cole1 , dihydroxyacetone phosphate , glycolysis , gene , enzyme , phenotype

The Clarke-Carbon clone bank carrying ColE1-Escherichia coli DNA has been screened by conjugation for complementation of glycolysis and hexose monophosphate shunt mutations. Plasmids were identified for phosphofructokinase (pfkA), triose phosphate isomerase (tpi), phosphoglucose isomerase (pgi), glucose-6-phosphate dehydrogenase (zwf), gluconate-6-phosphate dehydrogenase (gnd), enolase (eno), phosphoglycerate kinase (pgk), and fructose-1,6-P2 aldolase (fda). Enzyme levels for the plasmid-carried gene ranged, for the various plasmids, from 4- to 25-fold the normal level.

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