Open AccessCompact Escherichia coli nucleoids in a highly supercoiled conformation
Author(s) -
E C Materman,
A. P. Van Gool
Publication year - 1978
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.135.2.703-706.1978
Subject(s) - nucleoid , escherichia coli , dna supercoil , biology , rna , biophysics , dna , escherichia coli proteins , enterobacteriaceae , biochemistry , microbiology and biotechnology , gene , dna replication
Escherichia coli nucleoids were visualized in a compact, highly supercoiled conformation at 1 M NaCl. After the salt concentration was lowered to 0.15 M NaCl, the nucleoids uncoiled in beaded fibers in which RNA-mediated structural domains could be distinguished.
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