Regulation of the synthesis of ribonucleoside diphosphate reductase in Escherichia coli: specific activity of the enzyme in relationship to perturbations of DNA replication | Zendy

D Filpula | Zendy; J A Fuchs | Zendy

Open Access

Regulation of the synthesis of ribonucleoside diphosphate reductase in Escherichia coli: specific activity of the enzyme in relationship to perturbations of DNA replication

Author(s) -

D Filpula,

J A Fuchs

Publication year - 1978

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.135.2.429-435.1978

Subject(s) - dnaa , ribonucleotide reductase , biology , dna replication , escherichia coli , mutant , dna synthesis , biochemistry , reductase , dnag , enzyme , ribonucleoside , dna , dnab helicase , microbiology and biotechnology , eukaryotic dna replication , rna , protein subunit , helicase , gene

Ribonucleoside diphosphate reductase (RDP reductase) activity was found to greatly increase after a shift to the nonpermissive temperature in Escherichia coli mutants temperature sensitive for DNA elongation (dnaE dnaG dnaZ lig) or DNA initiation (dnaA dnaC dnaI). However, the kinetics of increase in RDP reductase after a shift to nonpermissive conditions were significantly different in initiation-defective mutants compared with elongation-defective mutants. In strains without defects in DNA metabolism, the specific activity of RDP reductase was found to increase with increasing growth rate. Nutritional shifts to faster growth conditions caused cells to transiently overproduce RDP reductase before adjusting to the new steady-state conditions.

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