Open AccessPolyamines in trypanosomatids
Author(s) -
Cyrus J. Bacchi,
Gregg Y. Lipschik,
Henry C. Nathan
Publication year - 1977
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.131.2.657-661.1977
Subject(s) - spermidine , biology , putrescine , crithidia fasciculata , spermine , trypanosoma brucei , crithidia , biochemistry , trypanosoma , polyamine , botany , enzyme , protozoa , genetics , gene
Polyamines were determined by n-butanol extraction and thin-layer chromatography in four trypanosomatids: Trypanosoma brucei (rat infection) and cultures of Crithidia fasciculata, Leptomonas sp., and Trypanosoma mega. All had putrescine and spermidine but no detectable spermine. Putrescine and spermidine levels were quantitated for extracts of leptomonas during the normal growth cycle. Spermidine values peaked 18 h before peak cell populations. Spermidine-putrescine ratios for all organisms were related to the presumed phylogeny of the group.
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