Open AccessProperties of RP4, an R Factor Which Originated in Pseudomonas aeruginosa S8
Author(s) -
J. R. Saunders,
J. Grinsted
Publication year - 1972
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.112.2.690-696.1972
Subject(s) - carbenicillin , biology , pseudomonas aeruginosa , tetracycline , extrachromosomal dna , kanamycin , cytosine , dna , guanine , microbiology and biotechnology , neomycin , genetics , escherichia coli , antibiotics , plasmid , gene , bacteria , nucleotide
RP4, an R factor derived fromPseudomonas aeruginosa S8 and specifying resistance to carbenicillin, neomycin, kanamycin, and tetracycline, could be isolated as an extrachromosomal satellite of covalently closed circular DNA (CCC-DNA) of molecular weight about 62 million and of buoyant density 1.719 g/cm3 (60% guanine plus cytosine). When RP4 was compared by hybridization with RP1, an R factor originating in another strain ofP. aeruginosa , it was shown that RP4 contains most, if not all, of the base sequences of RP1, and also additional sequences not found in RP1.
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