Open AccessDirect RNA Nanopore Sequencing of Pseudomonas aeruginosa Clone C Transcriptomes
Author(s) -
Marie-Madlen Pust,
Colin Davenport,
Lutz Wiehlmann,
Burkhard Tümmler
Publication year - 2022
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00418-21
Subject(s) - biology , rna , antisense rna , genetics , gene , small rna , genome , non coding rna , computational biology
Genome-wide gene expression of bacteria is commonly studied by high-throughput sequencing of size-selected cDNA fragment libraries of reverse-transcribed RNA preparations. However, the depletion of rRNAs, enzymatic reverse transcription, and the fragmentation, size selection, and amplification during library preparation lead to inevitable losses of information about the initial composition of the RNA pool.