Open AccessA Practical Guide to Small Protein Discovery and Characterization Using Mass Spectrometry
Author(s) -
Christian H. Ahrens,
Joseph T. Wade,
Matthew M. Champion,
Julian D. Langer
Publication year - 2022
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00353-21
Subject(s) - mass spectrometry , biology , computational biology , proteomics , protein mass spectrometry , mass spectrometry imaging , tandem mass tag , small molecule , isobaric labeling , bioinformatics , biochemistry , tandem mass spectrometry , quantitative proteomics , chromatography , chemistry , gene
Small proteins of up to ∼50 amino acids play important physiological roles across all domains of life. Mass spectrometry is an ideal approach to detect and characterize small proteins, but many aspects of standard mass spectrometry workflows are biased against small proteins due to their size. Here, we highlight applications of mass spectrometry to study small proteins, emphasizing modifications to standard workflows to optimize the detection of small proteins.