Open AccessVacuole Acidification Is Not Required for Survival of Salmonella enterica Serovar Typhimurium within Cultured Macrophages and Epithelial Cells
Author(s) -
Olivia SteeleMortimer,
Maryse StLouis,
Martin Olivier,
B. Brett Finlay
Publication year - 2000
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.68.9.5401-5404.2000
Subject(s) - bafilomycin , phagosome , vacuole , salmonella enterica , intracellular , biology , microbiology and biotechnology , cell culture , salmonella , macrophage , intracellular parasite , cytoplasm , biochemistry , bacteria , autophagy , in vitro , apoptosis , genetics
Phagosome acidification is an important component of the microbicidal response by infected eukaryotic cells. Thus, intracellular pathogens that reside within phagosomes must either block phagosome acidification or be able to survive at low pH. In this work, we studied the effect of phagosomal acidification on the survival of intracellularSalmonella enterica serovar Typhimurium in different cell types. Bafilomycin A1, a specific inhibitor of the vacuolar proton-ATPases, was used to block acidification of salmonella-containing vacuoles. We found that in several epithelial cell lines, treatment with bafilomycin A1 had no effect on intracellular survival or replication. Furthermore, although acidification was essential forSalmonella intracellular survival in J774 cultured macrophages, as reported previously (13), it is not essential in other macrophage cell lines. These data suggest that vacuolar acidification may play a role in intracellular survival of salmonellae only under certain conditions and in specific cell types.