Open AccessHumoral and cellular immunity to hepatitis B virus-derived antigens: comparative activity of Freund complete adjuvant alum, and liposomes
Author(s) -
Yanuario Sanchez,
Irina IonescuMatiu,
Gordon R. Dreesman,
William Kramp,
Howard R. Six,
F. Blaine Hollinger,
J L Melnick
Publication year - 1980
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.30.3.728-733.1980
Subject(s) - immunogenicity , adjuvant , hbsag , cellular immunity , humoral immunity , immune system , liposome , antigen , alum , virology , immunity , biology , immunology , microbiology and biotechnology , virus , hepatitis b virus , chemistry , biochemistry , organic chemistry
Complete Freud adjuvant, aluminum gel, and liposomes were compared for their ability to enhance the immunogenicity of an intact 22-nm HBsAg particle vaccine and an HBsAg-derived polypeptide vaccine in guinea pigs. Both humoral and cell-mediated immune responses were evaluated. The greatest immune response was obtained with complete Freund adjuvant, regardless of the antigen preparation. Aluminum gel appeared to be a better adjuvant for 22-nm HBsAg particles, but the liposomes rendered polypeptide preparations more immunogenic. The possibility that various proportions were entrapped in aqueous compartments instead of being inserted into the lipid bilayers of liposomes might account for this difference. The development of both humoral and cellular immunity was dependent upon the use of an adjuvant, because aqueous preparations had poor immunogenicity.