Open AccessVirus replication and localization of varicella-zoster virus antigens in human embryonic fibroblast cells infected with cell-free virus
Author(s) -
Koichi Yamanishi,
Y Matsunaga,
Takeo Ogino,
M Takahashi,
Akihisa Takamizawa
Publication year - 1980
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.28.2.536-541.1980
Subject(s) - biology , virology , virus , cytoplasm , antigen , varicella zoster virus , viral replication , fibroblast , cell nucleus , microbiology and biotechnology , cell culture , genetics
When human embryonic fibroblast cells were infected with cell-free varicella-zoster virus, virus replication began between 8 and 14 h postinfection, and 4 more h werp required for the virus to infect neighboring cells. Virus-specific antigens were traced by the anticomplement immunofluorescent antibody technique. Virus antigen was first detectable 2 h postinfection in the cytoplasma, and diffuse fluorescence was observed in the nucleus as early as 4 h after infection. The nuclear fluorescence got brighter and cytoplasmic fluorescence was observed at 14 h postinfection. The spread of virus to the neighboring cells was recognized in 18 h postinfection. In the period of 24 to 48 h, antigens were seen at the nuclear membrane region and in the cytoplasma. Very strong fluorescence was restricted mainly to the nucleus, when phosphonoacetic acid or cytosine arabinoside was added to the infected cultures and the cells were incubated for 48 h.