Open AccessProperties of an Escherichia coli cytotoxin
Author(s) -
Jack Konowalchuk,
N. Dickie,
S. Stavrić,
Joan I. Speirs
Publication year - 1978
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.20.2.575-577.1978
Subject(s) - escherichia coli , biology , isoelectric focusing , pi , vero cell , microbiology and biotechnology , enterobacteriaceae , bacteria , cytotoxic t cell , molecular mass , biochemistry , gene , enzyme , in vitro , genetics
Isoelectric focusing of a heat-labile cytotoxin of Escherichia coli H30 revealed the presence of two molecular variants, pI 7.2 and a comparatively small quantity of pI 6.8. Predominant component pI 7.2 had a molecular weight of 28,000, induced some fluid accumulation in rabbit ileal loops, and showed no morphological response in Y-1 cells but a strong cytotoxic effect on Vero cells.
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