Open AccessPlaque Formation by Chlamydia in L Cells
Author(s) -
Joyce Banks,
B. Eddie,
Julius Schachter,
K. F. Meyer
Publication year - 1970
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.1.3.259-262.1970
Subject(s) - chlamydiae , infectivity , biology , virus quantification , microbiology and biotechnology , chlamydia , trachoma , chlamydia trachomatis , virology , chlamydiaceae , cell culture , fibroblast , subculture (biology) , immunology , pathology , virus , medicine , genetics
Chlamydiae were found capable of producing plaques in several cell lines. Mouse fibroblast cells, L-929, proved the most sensitive to infection and yielded plaques of the highest clarity. Assay of chlamydial infectivity by plaque titration was at least as sensitive as egg ld(50) determination. Among chlamydial isolates of avian, mammalian, and human origin, only slow-growing trachoma-inclusion-conjunctivitis agents did not produce plaques. The plaque assay is highly sensitive, reproducible, and offers a potential tool for investigations requiring accurate measurement of small changes in chlamydial infectivity.