Open AccessPurification and Characterization of Two Proteins from Culture Filtrates of Mycobacterium tuberculosis H 37 Ra Strain
Author(s) -
Thomas M. Daniel,
Lavenia E. Ferguson
Publication year - 1970
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.1.2.164-168.1970
Subject(s) - mycobacterium tuberculosis , antiserum , strain (injury) , antigen , biology , size exclusion chromatography , tuberculosis , microbiology and biotechnology , gel electrophoresis , molecular mass , mycobacterium , biochemistry , bacteria , immunology , enzyme , genetics , medicine , pathology , anatomy
Two proteins have been purified from culture filtrates of Mycobacterium tuberculosis, H(37)Ra strain by a procedure combining gel filtration, diethylaminoethyl (DEAE)-cellulose chromatography, and zone electrophoresis. The two proteins are similar in molecular weight but differ slightly in charge. The faster migrating protein, designated a(1), is not antigenic. The slower migrating protein, designated a(2), is antigenic both with respect to antisera and as a skin-testing antigen.