Open AccessOsmotically Sensitive Brucella in Infected Normal and Immune Macrophages
Author(s) -
Jerry R. McGhee,
Bob A. Freeman
Publication year - 1970
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.1.2.146-150.1970
Subject(s) - brucella , biology , immune system , guinea pig , microbiology and biotechnology , brucella abortus , intracellular , macrophage , antiserum , phagocytosis , immunology , antigen , brucellosis , in vitro , biochemistry , endocrinology
When Brucella suis is grown in tissue cultures of normal guinea pig macrophages, the Brucella multiplies significantly without the induction of osmotically sensitive forms. In immune macrophages in the presence of normal guinea pig serum, there is a reduction in the number of intracellular Brucella and no multiplication is seen over a 72-hr period. After 6 hr of exposure to immune macrophages, however, approximately 50% of the surviving intracellular Brucella are osmotically sensitive, i.e., they will survive and grow only on medium containing 0.2 m sucrose. Brucella grown in immune macrophages, in the presence of rabbit antiserum against whole guinea pig serum, show the presence of osmotically sensitive forms, although at a reduced level compared to the number seen with immune macrophages in normal guinea pig serum.