Open AccessResistance Factor-Mediated Spectinomycin Resistance
Author(s) -
David H. Smith,
J. A. Janjigian,
Naomi Prescott,
Patrick Anderson
Publication year - 1970
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.1.1.120-127.1970
Subject(s) - spectinomycin , streptomycin , biology , microbiology and biotechnology , enteric bacteria , enzyme , drug resistance , biochemistry , antibiotics , escherichia coli , gene
Of 100 natural isolates of drug-resistant enteric bacteria, 51 were resistant to spectinomycin (Spc) and 46 contained transferable R factors mediating Spc resistance. All Spc(R) R factors mediated streptomycin and bluensomycin resistance and were fi(+) type. Extracts of R-Spc(R) strains adenylated Spc, dihydrospectinomycin, actinamine, streptomycin, and bluensomycin in vitro in the presence of adenosine triphosphate and Mg(++). Results of genetic and biochemical studies support the hypothesis that these reactions are mediated by a single enzyme.