Open AccessTaurine-Mediated IDOL Contributes to Resolution of Streptococcus uberis Infection
Author(s) -
Zhixin Wan,
Riguo Lan,
Yifei Zhou,
Yuanyuan Xu,
Zhenglei Wang,
Zhenhua Luo,
Jinfeng Miao
Publication year - 2021
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.00788-20
Subject(s) - biology , microbiology and biotechnology , streptococcus uberis , pathogen , lipid metabolism , bacteria , lipogenesis , mastitis , taurine , pathogenic bacteria , lipid droplet , streptococcus , lipid a , biochemistry , amino acid , genetics
Metabolic alterations occur in pathogenic infections, but the role of lipid metabolism in the progression of bacterial mastitis is unclear. Cross talk between lipid droplets (LDs) and invading bacteria occurs, and targeting of de novo lipogenesis inhibits pathogen reproduction. In this study, we investigate the role(s) of lipid metabolism in mammary cells during Streptococcus uberis infection. Our results indicate that S. uberis induces the synthesis of fatty acids and production of LDs. Importantly, taurine reduces fatty acid synthesis, the abundance of LDs and the in vitro bacterial load of S. uberis These changes are mediated, at least partly, by the E3 ubiquitin ligase IDOL, which is associated with the degradation of low-density lipoprotein receptors (LDLRs). We have identified a critical role for IDOL-mediated fatty acid synthesis in bacterial infection, and we suggest that taurine may be an effective prophylactic or therapeutic strategy for preventing S. uberis mastitis.