Open AccessB Cell Compartmentalization in Blood and Cerebrospinal Fluid of HIV-Infected Ugandans with Cryptococcal Meningitis
Author(s) -
Samuel Okurut,
David B. Meya,
Freddie Bwanga,
Joseph Olobo,
Michael A. Eller,
Fatim Cham-Jallow,
Paul R. Bohjanen,
Harsh Pratap,
Brent E. Palmer,
Katharine H. Hullsiek,
Yukari C. Manabe,
David R. Boulware,
Edward N. Janoff
Publication year - 2020
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.00779-19
Subject(s) - biology , cryptococcal meningitis , compartmentalization (fire protection) , cerebrospinal fluid , virology , meningitis , immunology , cryptococcosis , human immunodeficiency virus (hiv) , microbiology and biotechnology , viral disease , medicine , neuroscience , psychiatry , biochemistry , enzyme
Activated B cells modulate infection by differentiating into pathogen-specific antibody-producing effector plasmablasts/plasma cells, memory cells, and immune regulatory B cells. In this context, the B cell phenotypes that infiltrate the central nervous system during human immunodeficiency virus (HIV) and cryptococcal meningitis coinfection are ill defined. We characterized clinical parameters, mortality, and B cell phenotypes in blood and cerebrospinal fluid (CSF) by flow cytometry in HIV-infected adults with cryptococcal ( n = 31) and noncryptococcal ( n = 12) meningitis and in heathy control subjects with neither infection ( n = 10). Activation of circulating B cells (CD21 low ) was significantly higher in the blood of subjects with HIV infection than in that of healthy controls and greater yet in matched CSF B cells ( P < 0.001). Among B cell subsets, elevated frequencies of memory and plasmablasts/plasma cells most clearly distinguished the CSF from blood compartments. With cryptococcal meningitis, lower frequencies of expression of the regulatory protein programmed death-1 (PD-1) on plasmablasts/plasma cells in blood (median, 7%) at presentation were associated with significantly decreased 28-day survival (29% [4/14 subjects]), whereas higher PD-1 expression (median, 46%) characterized subjects with higher survival (88% [14/16 subjects]). With HIV infection, B cell differentiation and regulatory markers are discrete elements of the circulating and CSF compartments with clinical implications for cryptococcal disease outcome, potentially due to their effects on the fungus and other local immune cells.