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Presented here is the complete genome sequence of the well-studied Rhizobiales methanotroph Methylosinus trichosporium strain OB3b. The assembly contains 5,183,433 bp, corresponding to a chromosome of 4,508,832 bp and three circular plasmids of 285,280 bp, 209,102 bp, and 180,219 bp.

The Completed PacBio Single-Molecule Real-Time Sequence of Methylosinus trichosporium Strain OB3b Reveals the Presence of a Third Large Plasmid