Open AccessComplete Genome Sequence of a New Tobamovirus Naturally Infecting Tomatoes in Mexico
Author(s) -
Rugang Li,
Shan Gao,
Zhangjun Fei,
KaiShu Ling
Publication year - 2013
Publication title -
genome announcements
Language(s) - English
Resource type - Journals
ISSN - 2169-8287
DOI - 10.1128/genomea.00794-13
Subject(s) - tobamovirus , biology , sanger sequencing , phylogenetic tree , whole genome sequencing , sequence (biology) , genetics , genome , complete sequence , dna sequencing , virology , computational biology , gene , rna
The complete genomic sequence of a new tobamovirus in tomatoes was determined through deep sequencing and assembly of small RNAs, then validated through Sanger sequencing. Based on the low sequence identity (≤85%) to known viruses and a close phylogenetic relationship to tobamoviruses, it was identified as a new species.