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Metagenome Sequencing Reveals Rhodococcus Dominance in Farpuk Cave, Mizoram, India, an Eastern Himalayan Biodiversity Hot Spot Region
Author(s) -
Surajit De Mandal,
Zothan Sanga,
Nachimuthu Senthil Kumar
Publication year - 2015
Publication title -
genome announcements
Language(s) - English
Resource type - Journals
ISSN - 2169-8287
DOI - 10.1128/genomea.00610-15
Subject(s) - biology , metagenomics , cave , phylum , 16s ribosomal rna , dominance (genetics) , biodiversity , rhodococcus , amplicon , microbiome , ribosomal rna , ecology , evolutionary biology , zoology , bacteria , microbiology and biotechnology , genetics , gene , polymerase chain reaction
The present study employed 16S rRNA amplicon sequencing to survey the prokaryotic microbiota on Farpuk Cave, revealing a diverse bacterial community with 4,021 operational taxonomical units (OTUs), mainly dominated by the genus Rhodococcus. Moreover, 18.17% of the OTUs were unclassified at the phylum level, suggesting the existence of novel bacterial species.

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