Complete Genome Sequence of Enterovirus D68 Detected in Classroom Air and on Environmental Surfaces
Author(s) -
John A. Lednicky,
Tania S. Bonny,
J. Glenn Morris,
Julia C. Loeb
Publication year - 2016
Publication title -
genome announcements
Language(s) - English
Resource type - Journals
ISSN - 2169-8287
DOI - 10.1128/genomea.00579-16
Subject(s) - genome , biology , enterovirus , sampling (signal processing) , whole genome sequencing , transcription (linguistics) , air filter , genetics , filter (signal processing) , gene , virus , computer science , engineering , mechanical engineering , inlet , computer vision , linguistics , philosophy
We amplified and sequenced the complete genome of enterovirus D68 (EV-D68) that had been collected from classroom air using a filter-based air sampling method and by swab sampling of environmental surfaces. Relatively high levels of EV-D68 genome equivalents were found per cubic meter of air by quantitative real-time reverse transcription-PCR (RT-PCR).
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