Open AccessComplete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader
Author(s) -
Yoshiyuki Ohtsubo,
Shouta oyama,
Yasunobu Nagata,
Masahiro Numata,
Keiko Tsuchikane,
Akira Hosoyama,
Atsushi Yamazoe,
Masashi Tsuda,
Nobuyuki Fujita,
Fusako Kawai
Publication year - 2016
Publication title -
genome announcements
Language(s) - English
Resource type - Journals
ISSN - 2169-8287
DOI - 10.1128/genomea.00529-16
Subject(s) - polyethylene glycol , whole genome sequencing , strain (injury) , biology , genome , nucleotide , genetics , computational biology , gene , biochemistry , anatomy
We determined the complete genome sequence of Sphingopyxis macrogoltabida strain 203N, a polyethylene glycol degrader. Because the PacBio assembly (285× coverage) seemed to be full of nucleotide-level mismatches, the Newbler assembly of MiSeq mate-pair and paired-end data was used for finishing and the PacBio assembly was used as a reference. The PacBio assembly carried 414 nucleotide mismatches over 5,953,153 bases of the 203N genome.