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We report here the draft genome sequence of Alicycliphilus sp. B1, isolated from activated sludge in a wastewater treatment plant of an electronic component factory as an N-acylhomoserine lactone-producing strain. The draft genome is 7,465,959 bp in length, with 59 large contigs. About 7,391 protein-coding genes, 82 tRNAs, and 13 rRNAs are predicted from this assembly.

Draft Genome Sequence of Alicycliphilus sp. B1, an N -Acylhomoserine Lactone-Producing Bacterium, Isolated from Activated Sludge