Structure and Genome Organization of Cherry Virus A ( Capillovirus, Betaflexiviridae ) from China Using Small RNA Sequencing | Zendy

Jiawei Wang | Zendy; Ying Zhai | Zendy; Weizhen Liu | Zendy; Amit Dhingra | Zendy; H. R. Pappu | Zendy; Qingzhong Liu | Zendy

Open Access

Structure and Genome Organization of Cherry Virus A ( Capillovirus, Betaflexiviridae ) from China Using Small RNA Sequencing

Author(s) -

Jiawei Wang,

Ying Zhai,

Weizhen Liu,

Amit Dhingra,

H. R. Pappu,

Qingzhong Liu

Publication year - 2016

Publication title -

genome announcements

Language(s) - English

Resource type - Journals

ISSN - 2169-8287

DOI - 10.1128/genomea.00364-16

Subject(s) - biology , untranslated region , genome , rna , genetics , reverse transcriptase , deep sequencing , complementary dna , gene , computational biology , virology

Cherry virus A (CVA) (Capillovirus, Betaflexiviridae) is widely present in cherry-growing areas. We obtained the complete genome of a CVA isolate (CVA-TA) using small RNA deep sequencing, followed by overlapping reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). The newly identified 5'-untranslated region (5'-UTR) from CVA-TA may form additional hairpin and loop structures to stabilize the CVA genome.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research