Open AccessGenome Resequencing of the Virulent and Multidrug-Resistant Reference Strain Clostridium difficile 630
Author(s) -
Thomas Riedel,
Boyke Bunk,
Andrea Thürmer,
Cathrin Spröer,
Elżbieta Brzuszkiewicz,
Birte Abt,
Sabine Gronow,
Heiko Liesegang,
Rolf Daniel,
Jörg Overmann
Publication year - 2015
Publication title -
genome announcements
Language(s) - English
Resource type - Journals
ISSN - 2169-8287
DOI - 10.1128/genomea.00276-15
Subject(s) - biology , virulence , clostridium difficile , transposable element , genome , whole genome sequencing , strain (injury) , genetics , gene , illumina dye sequencing , multiple drug resistance , pathogen , microbiology and biotechnology , drug resistance , antibiotics , anatomy
We resequenced the complete genome of the virulent and multidrug-resistant pathogen Clostridium difficile strain 630. A combination of single-molecule real-time and Illumina sequencing technology revealed the presence of an additional rRNA gene cluster, additional tRNAs, and the absence of a transposon in comparison to the published and reannotated genome sequence.