Open AccessThe Highly Conserved tRNA His Guanylyltransferase Thg1p Interacts with the Origin Recognition Complex and Is Required for the G 2 /M Phase Transition in the Yeast Saccharomyces cerevisiae
Author(s) -
Terri S. Rice,
Min Ding,
David S. Pederson,
Nicholas H. Heintz
Publication year - 2005
Publication title -
eukaryotic cell
Language(s) - English
Resource type - Journals
eISSN - 1535-9778
pISSN - 1535-9786
DOI - 10.1128/ec.4.4.832-835.2005
Subject(s) - saccharomyces cerevisiae , biology , cytokinesis , yeast , origin recognition complex , transfer rna , microbiology and biotechnology , cell division , mutant , genetics , dna replication , dna , gene , cell , rna , eukaryotic dna replication
Here we show that the Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p interacts with the origin recognition complex in vivo and in vitro and that overexpression of hemagglutinin-Thg1p selectively impedes growth of orc2-1(Ts) cells at the permissive temperature. Studies with conditional mutants indicate that Thg1p couples nuclear division and migration to cell budding and cytokinesis in yeast.