Open AccessTranslational Accuracy during Exponential, Postdiauxic, and Stationary Growth Phases in Saccharomyces cerevisiae
Author(s) -
Guillaume Stahl,
Samia N. Ben Salem,
Lifeng Chen,
Bing Zhao,
Philip J. Farabaugh
Publication year - 2004
Publication title -
eukaryotic cell
Language(s) - English
Resource type - Journals
eISSN - 1535-9778
pISSN - 1535-9786
DOI - 10.1128/ec.3.2.331-338.2004
Subject(s) - saccharomyces cerevisiae , translation (biology) , translational regulation , biology , exponential growth , yeast , translational efficiency , protein biosynthesis , microbiology and biotechnology , eukaryotic translation , messenger rna , genetics , biochemistry , gene , physics , quantum mechanics
When the yeast Saccharomyces cerevisiae shifts from rapid growth on glucose to slow growth on ethanol, it undergoes profound changes in cellular metabolism, including the destruction of most of the translational machinery. We have examined the effect of this metabolic change, termed the diauxic shift, on the frequency of translational errors. Recoding sites are mRNA sequences that increase the frequency of translational errors, providing a convenient reporter of translational accuracy. We found that the diauxic shift causes no overall change in translational accuracy but does cause a strong reduction in the frequency of one type of programmed error: Ty +1 frameshifting. Genetic data suggest that this effect may be due to changes in the relative amounts of tRNA participating in translation elongation. We discuss possible implications for expression strategies that use recoding.