Physiological and Biochemical Characterization of AnNitA, the Aspergillus nidulans High-Affinity Nitrite Transporter | Zendy

Shiela E. Unkles | Zendy; Vicki F. Symington | Zendy; Zorica Kotur | Zendy; Ye Wang | Zendy; M. Yaeesh Siddiqi | Zendy; James R. Kinghorn | Zendy; Anthony D. M. Glass | Zendy

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Physiological and Biochemical Characterization of AnNitA, the Aspergillus nidulans High-Affinity Nitrite Transporter

Author(s) -

Shiela E. Unkles,

Vicki F. Symington,

Zorica Kotur,

Ye Wang,

M. Yaeesh Siddiqi,

James R. Kinghorn,

Anthony D. M. Glass

Publication year - 2011

Publication title -

eukaryotic cell

Language(s) - English

Resource type - Journals

eISSN - 1535-9778

pISSN - 1535-9786

DOI - 10.1128/ec.05199-11

Subject(s) - aspergillus nidulans , asparagine , transporter , biology , nitrite , biochemistry , kinetics , enzyme kinetics , enzyme , gene , active site , physics , ecology , quantum mechanics , mutant , nitrate

High-affinity nitrite influx into mycelia ofAspergillus nidulans has been characterized by use of13 NO2 − , giving averageKm andV max values of 48 ± 8 μM and 228 ± 49 nmol mg−1 dry weight (DW) h−1 , respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (r 2 = 0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the possible roles of conserved asparagine residues N122 (transmembrane domain 3 ]Tm 3]), N173 (Tm 4), N214 (Tm 5), and N246 (Tm 6) are discussed.

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