Open AccessEffect of Hydroxyurea on Procyclic Trypanosoma brucei : an Unconventional Mechanism for Achieving Synchronous Growth
Author(s) -
Arnab Roy Chowdhury,
Zhixing Zhao,
Paul T. Englund
Publication year - 2008
Publication title -
eukaryotic cell
Language(s) - English
Resource type - Journals
eISSN - 1535-9778
pISSN - 1535-9786
DOI - 10.1128/ec.00369-07
Subject(s) - trypanosoma brucei , kinetoplast , minicircle , biology , microbiology and biotechnology , dna replication , dna , genetics , gene
ProcyclicTrypanosoma brucei cells were synchronized with 0.2 mM hydroxyurea. The cells did not arrest at the G1 /S boundary but proceeded through one round of replication and arrested near the end of S phase. The mitochondrial genome (kinetoplast DNA network) replicated, forming two progeny networks, but the repair of minicircle gaps was inhibited.