Open AccessUse of the Kinase Inhibitor Analog 1NM-PP1 Reveals a Role for Toxoplasma gondii CDPK1 in the Invasion Step
Author(s) -
Tatsuki Sugi,
Kentaro Kato,
Kanae Kobayashi,
Shumpei Watanabe,
Hitomi Kurokawa,
Haiyan Gong,
Kishor Pandey,
Hitoshi Takemae,
Hiroomi Akashi
Publication year - 2010
Publication title -
eukaryotic cell
Language(s) - English
Resource type - Journals
eISSN - 1535-9778
pISSN - 1535-9786
DOI - 10.1128/ec.00351-09
Subject(s) - toxoplasma gondii , motility , biology , secretion , glycine , gliding motility , kinase , microbiology and biotechnology , biochemistry , amino acid , genetics , antibody
Toxoplasma gondii CDPK1 (TgCDPK1) was found to be the target of the toxoplasmocidal compound 1NM-PP1. When TgCDPK1 was mutated at position 128 from glycine to methionine, resistance was gained. Inhibition of gliding motility without inhibition of micronemal secretion by 1NM-PP1 suggests a function for TgCDPK1 in gliding motility.