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Here, we describe the isolation of two nickel-induced genes inParamecium caudatum ,NCI16 and PcGST1 , by subtractive hybridization.NCI16 encoded a predicted four-transmembrane domain protein (∼16 kDa) of unknown function, and PcGST1 encoded glutathioneS -transferase (GST; ∼25 kDa) with GST and glutathione peroxidase (GPx) activities. Exposing cells to cobalt chloride also caused the moderate upregulation ofNCI16 and PcGST1 mRNAs. Both nickel sulfate and cobalt chloride dose dependently inducedNCI16 and PcGST1 mRNAs, but with different profiles. Nickel treatment caused a continuous increase in PcGST1 andNCI16 mRNA levels for up to 3 and 6 days, respectively, and a notable increase in H2 O2 concentrations inP. caudatum .NCI16 expression was significantly enhanced by incubating cells with H2 O2 , implying thatNCI16 induction in the presence of nickel ions is caused by reactive oxygen species (ROS). On the other hand, PcGST1 was highly induced by the antioxidanttert -butylhydroquinone (tBHQ) but not by H2 O2 , suggesting that different mechanisms mediate the induction ofNCI16 and PcGST1 . We introduced a luciferase reporter vector with an ∼0.42-kb putative PcGST1 promoter into cells and then exposed the transformants to nickel sulfate. This resulted in significant luciferase upregulation, indicating that the putative PcGST1 promoter contains a nickel-responsive element. Our nickel-inducible system also may be applicable to the efficient expression of proteins that are toxic to host cells or require temporal control.

Identification of Two Nickel Ion-Induced Genes, NCI16 and Pc GST1 , in Paramecium caudatum