Open AccessA New Assay for Promoter Analysis in Chlamydomonas Reveals Roles for Heat Shock Elements and the TATA Box in HSP70A Promoter-Mediated Activation of Transgene Expression
Author(s) -
Mukesh Lodha,
Miriam SchulzRaffelt,
Michael Schroda
Publication year - 2008
Publication title -
eukaryotic cell
Language(s) - English
Resource type - Journals
eISSN - 1535-9778
pISSN - 1535-9786
DOI - 10.1128/ec.00055-07
Subject(s) - tata box , promoter , biology , chlamydomonas , transgene , caat box , promoter activity , microbiology and biotechnology , gene expression , heat shock , gene , genetics , heat shock protein , mutant
The aim of this work was to identifycis -regulatory sequences within theChlamydomonas HSP70A promoter that mediate its stimulatory effect on the expression of downstream promoters. For this, we deleted/mutated theHSP70A promoter and, using a new assay, quantified its stimulatory effect. Our results indicate that the effect is mediated largely by heat shock elements and the TATA box.