Open AccessEvidence of a Continuous Endoplasmic Reticulum in the Protozoan Parasite Entamoeba histolytica
Author(s) -
José E. Teixeira,
Christopher D. Huston
Publication year - 2008
Publication title -
eukaryotic cell
Language(s) - English
Resource type - Journals
eISSN - 1535-9778
pISSN - 1535-9786
DOI - 10.1128/ec.00007-08
Subject(s) - endoplasmic reticulum , entamoeba histolytica , biology , fluorescence recovery after photobleaching , green fluorescent protein , entamoeba , microbiology and biotechnology , vesicle , biochemistry , gene , membrane
Entamoeba histolytica , the cause of amebiasis, is believed to have no continuous endoplasmic reticulum (ER), with ER functions occurring in vesicles. Here, using an ER-targeted green fluorescent protein fusion protein and fluorescence loss in photobleaching, we have unambiguously demonstrated the presence of a continuous ER compartment in livingE. histolytica trophozoites.