Open AccessCcr4 Promotes Resolution of the Endoplasmic Reticulum Stress Response during Host Temperature Adaptation in Cryptococcus neoformans
Author(s) -
Virginia E. Havel,
Nathan K. Wool,
David Ayad,
Kurtis M. Downey,
Christabel F. Wilson,
Peter E. Larsen,
Julianne T. Djordjevic,
John C. Panepinto
Publication year - 2011
Publication title -
eukaryotic cell
Language(s) - English
Resource type - Journals
eISSN - 1535-9778
pISSN - 1535-9786
DOI - 10.1128/ec.00006-11
Subject(s) - cryptococcus neoformans , endoplasmic reticulum , unfolded protein response , biology , adaptation (eye) , mutant , microbiology and biotechnology , host (biology) , gene expression , virulence , fungal protein , gene , genetics , neuroscience
Adaptation to host temperature is a prerequisite for any pathogen capable of causing deep infection in humans. Our previous studies demonstrated that aCryptococcus neoformans ccr4 Δ mutant lacking the major deadenylase involved in regulated mRNA decay was defective in host temperature adaptation and therefore virulence. In this study, theccr4 Δ mutant was found to exhibit characteristics of chronic unfolded-protein response (UPR) engagement in both the gene expression profile and phenotype. We demonstrate that host temperature adaptation inC. neoformans is accompanied by transient induction of the endoplasmic reticulum (ER) stress response and that Ccr4-dependent posttranscriptional gene regulation contributes to resolution of ER stress during host temperature adaptation.