A Simple Manual Rosetting Method for Absolute CD4 + Lymphocyte Counting in Resource-Limited Countries | Zendy

Phonethipsavanh Nouanthong | Zendy; Supansa Pata | Zendy; Thira Sirisanthana | Zendy; Watchara Kasinrerk | Zendy

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A Simple Manual Rosetting Method for Absolute CD4 ⁺ Lymphocyte Counting in Resource-Limited Countries

Author(s) -

Phonethipsavanh Nouanthong,

Supansa Pata,

Thira Sirisanthana,

Watchara Kasinrerk

Publication year - 2006

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cvi.13.5.598-601.2006

Subject(s) - flow cytometry , monoclonal antibody , simple (philosophy) , lymphocyte subsets , chemistry , immunology , microbiology and biotechnology , computer science , antibody , biology , t cell , philosophy , immune system , epistemology

A CD4 monoclonal antibody which reacts with CD4+ lymphocytes but does not react significantly with monocytes was generated and used to develop a simple method for CD4 count. In a comparison with standard flow cytometry, high correlation was obtained. The developed method can be an alternative to flow cytometry for resource-limited countries.

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