Open AccessA Simple Manual Rosetting Method for Absolute CD4 + Lymphocyte Counting in Resource-Limited Countries
Author(s) -
Phonethipsavanh Nouanthong,
Supansa Pata,
Thira Sirisanthana,
Watchara Kasinrerk
Publication year - 2006
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.13.5.598-601.2006
Subject(s) - flow cytometry , monoclonal antibody , simple (philosophy) , lymphocyte subsets , chemistry , immunology , microbiology and biotechnology , computer science , antibody , biology , t cell , philosophy , immune system , epistemology
A CD4 monoclonal antibody which reacts with CD4+ lymphocytes but does not react significantly with monocytes was generated and used to develop a simple method for CD4 count. In a comparison with standard flow cytometry, high correlation was obtained. The developed method can be an alternative to flow cytometry for resource-limited countries.