Analysis of Defined Combinations of Monoclonal Antibodies in Anthrax Toxin Neutralization Assays and Their Synergistic Action | Zendy

Miriam M. Ngundi | Zendy; Bruce D. Meade | Zendy; Stephen F. Little | Zendy; Conrad P. Quinn | Zendy; Cindi R. Corbett | Zendy; Rebecca A. Brady | Zendy; Drusilla Burns | Zendy

Open Access

Analysis of Defined Combinations of Monoclonal Antibodies in Anthrax Toxin Neutralization Assays and Their Synergistic Action

Author(s) -

Miriam M. Ngundi,

Bruce D. Meade,

Stephen F. Little,

Conrad P. Quinn,

Cindi R. Corbett,

Rebecca A. Brady,

Drusilla Burns

Publication year - 2012

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cvi.05714-11

Subject(s) - bacillus anthracis , anthrax toxin , anthrax vaccines , antibody , monoclonal antibody , neutralization , toxin , virology , antigen , bivalent (engine) , microbiology and biotechnology , chemistry , biology , immunology , recombinant dna , immunization , bacteria , biochemistry , fusion protein , dna vaccination , genetics , gene , metal , organic chemistry

Antibodies against the protective antigen (PA) component of anthrax toxin play an important role in protection against disease caused by Bacillus anthracis . In this study, we examined defined combinations of PA-specific monoclonal antibodies for their ability to neutralize anthrax toxin in cell culture assays. We observed additive, synergistic, and antagonistic effects of the antibodies depending on the specific antibody combination examined and the specific assay used. Synergistic toxin-neutralizing antibody interactions were examined in more detail. We found that one mechanism that can lead to antibody synergy is the bridging of PA monomers by one antibody, with resultant bivalent binding of the second antibody. These results may aid in optimal design of new vaccines and antibody therapies against anthrax.

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