Open AccessUse of Commercial Enzyme Immunoassays To Detect Antibodies to the Herpes Simplex Virus Type 2 Glycoprotein G in a Low-Risk Population in Hanoi, Vietnam
Author(s) -
Thoại D. Ngô,
Oliver Laeyendecker,
Hanh La,
Wayne Hogrefe,
Rhoda Ashley Morrow,
Thomas C. Quinn
Publication year - 2008
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00437-06
Subject(s) - herpes simplex virus , concordance , virology , vietnamese , antibody , population , blot , biology , medicine , virus , immunology , environmental health , biochemistry , linguistics , philosophy , gene
Sera from 1,238 Vietnamese women in Hanoi were tested for herpes simplex virus type 2 (HSV-2). HSV-2 prevalence was 2.0%. The Kalon and Biokit assays showed significantly higher concordance to Western blotting data than did the Focus assay (P < 0.01). Screening by Focus and then retesting with Kalon/Biokit of positive samples can reduce falsely positive results significantly (P < 0.01).