Open AccessEvaluation of Salmonella -Vectored Campylobacter Peptide Epitopes for Reduction of Campylobacter jejuni in Broiler Chickens
Author(s) -
S. L. Layton,
M. J. Morgan,
Kimberly Cole,
Young Min Kwon,
Dan Donoghue,
Billy M. Hargis,
Neil R. Pumford
Publication year - 2011
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00379-10
Subject(s) - campylobacter jejuni , campylobacter , salmonella , biology , microbiology and biotechnology , broiler , epitope , immune system , immunology , antibody , bacteria , food science , genetics
Campylobacter is a leading cause of bacterial gastroenteritis in humans and is often linked to contaminated poultry products. LiveSalmonella vectors expressing three linear peptide epitopes fromCampylobacter proteins Cj0113 (Omp18/CjaD), Cj0982c (CjaA), and Cj0420 (ACE393) were administered to chicks by oral gavage on the day of hatch, and the chicks were challenged withCampylobacter jejuni on day 21. All three candidate vaccines produced consistent humoral immune responses with high levels of serum IgG and mucosal secretory IgA (sIgA), with the best response from the Cj0113 peptide-expressing vector.Campylobacter challenge following vaccination of three candidate vaccine groups decreasedCampylobacter recovery from the ileum compared to that for controls on day 32. The Cj0113 peptide-expressing vector reducedCampylobacter to below detectable levels. TheSalmonella -vectored Cj0113 subunit vaccine appears to be an excellent candidate for further evaluation as a tool for the reduction ofCampylobacter in poultry for improved food safety.