Open AccessElimination of False-Positive Results in a Luminex Assay for Pneumococcal Antibodies
Author(s) -
Jerry W. Pickering,
Matthew T. Larson,
Thomas B. Martins,
Susan S. Copple,
Harry R. Hill
Publication year - 2010
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00329-09
Subject(s) - antibody , bovine serum albumin , streptococcus pneumoniae , medicine , immunology , virology , chemistry , microbiology and biotechnology , biology , antibiotics
In our 14-valent Luminex assay for pneumococcal antibodies, we identified two groups of sera that caused false-positive results. The first group bound nonspecifically to the Luminex microspheres. The second group reacted specifically with bovine serum albumin (BSA). We describe here methods that eliminated the false-positive reactivity of both groups.