Open AccessDevelopment of a Rapid and Convenient Method for Determination of Rubella Virus-Specific Immunoglobulin G Avidity
Author(s) -
Christelle VauloupFellous,
Jessica Ursulet-Diser,
L. Grangeot-Keros
Publication year - 2007
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00312-07
Subject(s) - avidity , rubella virus , rubella , immunoglobulin g , virology , antibody , specific antibody , computer science , immunology , biology , vaccination , measles
We describe here a rapid and semiautomated method for the determination of rubella virus immunoglobulin G (IgG) avidity with the VIDAS instrument. A total of 153 serum samples from persons with naturally acquired rubella virus infections (n = 98), from vaccinated persons (n = 44), and from patients with autoantibodies (n = 11) were included in this study. The rubella virus-specific IgG avidity assay we developed for the VIDAS instrument was evaluated by comparison with an in-house method. Results obtained with the VIDAS instrument allow considering this method valuable to help confirm or exclude acute primary infection or recent vaccination.