Open AccessWestern Blot Assay Using Recombinant p26 Antigen for Detection of Equine Infectious Anemia Virus-Specific Antibodies
Author(s) -
Irene Álvarez,
Gerónimo Gutiérrez,
Eileen N. Ostlund,
M. Barrandeguy,
K. Trono
Publication year - 2007
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00293-07
Subject(s) - equine infectious anemia , virology , recombinant dna , western blot , antibody , antigen , immunodiffusion , virus , biology , microbiology and biotechnology , immunology , biochemistry , gene
We analyzed the performance of a single-band Western blot (WB) test using recombinant p26 (rp26) capsid protein of equine infectious anemia virus. According to the results obtained, the rp26 WB test is a reliable confirmatory diagnostic tool to be used as a complementary test after an enzyme-linked immunosorbent assay or agar gel immunodiffusion test yielding doubtful results.