Open AccessEnzyme-Linked Immunosorbent Assay Method for Detection of Cytomegalovirus Strain-Specific Antibody Responses
Author(s) -
Zdeněk Novák,
Shan A. Ross,
A. Raj Kumar Patro,
Sunil Pati,
Meera K. Reddy,
Misty Purser,
William J. Britt,
Suresh B. Boppana
Publication year - 2009
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00281-08
Subject(s) - cytomegalovirus , serology , antibody , virology , epitope , glycoprotein , human cytomegalovirus , enzyme , biology , microbiology and biotechnology , herpesviridae , immunology , virus , viral disease , biochemistry
Reliable methods for the detection of cytomegalovirus (CMV) strain-specific serological responses are lacking. We describe a simple and reliable enzyme-linked immunosorbent assay method developed to detect antibodies against the polymorphic epitopes within the two envelope glycoproteins of CMV, glycoproteins H and B. This assay is useful for the detection of serologic responses to CMV strains and the identification of CMV reinfections.