Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis | Zendy

Konstantin P. Lyashchenko | Zendy; Adrian Grandison | Zendy; Karen Keskinen | Zendy; Alina Sikar-Gang | Zendy; Paul Lambotte | Zendy; Javan Esfandiari | Zendy; Gregory C. Ireton | Zendy; Aarthy C. Vallur | Zendy; Steven G. Reed | Zendy; Gareth J. Jones | Zendy; H. M. Vordermeier | Zendy; Judy Stabel | Zendy; Tyler C. Thacker | Zendy; Mitchell V. Palmer | Zendy; W. Ray Waters | Zendy

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Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Author(s) -

Konstantin P. Lyashchenko,

Adrian Grandison,

Karen Keskinen,

Alina Sikar-Gang,

Paul Lambotte,

Javan Esfandiari,

Gregory C. Ireton,

Aarthy C. Vallur,

Steven G. Reed,

Gareth J. Jones,

H. M. Vordermeier,

Judy Stabel,

Tyler C. Thacker,

Mitchell V. Palmer,

W. Ray Waters

Publication year - 2017

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cvi.00259-17

Subject(s) - antigen , bovine tuberculosis , mycobacterium bovis , immunoassay , tuberculosis , antibody , virology , mycobacterium tuberculosis , biology , immunology , recombinant dna , medicine , biochemistry , pathology , gene

Bovine tuberculosis (TB), caused byMycobacterium bovis , remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquiredM. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

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