Open AccessExpression Profiles of Genes in Toll-Like Receptor-Mediated Signaling of Broilers Infected with Clostridium perfringens
Author(s) -
Yang Lü,
Aimie J. Sarson,
Joshua Gong,
Huaijun Zhou,
Weiyun Zhu,
Zhumei Kang,
Hai Yu,
Shayan Sharif,
Yanming Han
Publication year - 2009
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00254-09
Subject(s) - clostridium perfringens , tlr2 , trif , biology , toll like receptor , tlr4 , innate immune system , microbiology and biotechnology , downregulation and upregulation , immune system , tlr5 , tlr7 , tlr3 , signal transduction , tumor necrosis factor alpha , spleen , receptor , gene , immunology , genetics , bacteria
Toll-like receptors (TLRs) participate in detecting microbial pattern molecules for activation of the host immune response. We investigated possible roles of TLRs in the chicken response toClostridium perfringens infection by examining the expression of TLR genes and other genes involved in TLR-mediated signaling within the spleens and ilea ofC. perfringens -challenged broilers. Upregulation of a tumor necrosis factor alpha-inducing factor homolog in challenged chickens compared to naïve chickens was observed, regardless of the incidence of necrotic enteritis. In addition, the members of the TLR2 subfamily were found to be most strongly involved in the host response toC. perfringens challenge, although the expression of TLR4 and TLR7 was also upregulated in spleen tissues. While the combination of TLR1.2, TLR2.1, and TLR15 appeared to play a major role in the splenic response, the expression of TLR2.2 and TLR1.1 was positively correlated to the expression of adaptor molecules MyD88, TRAF6, TRIF, and receptor interacting protein 1 in the ileal tissues, demonstrating a dynamic spatial and temporal innate host response toC. perfringens .