Open AccessEvaluation of Dried Whole Blood Spots Obtained by Heel or Finger Stick as an Alternative to Venous Blood for Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Vertically Exposed Infants in the Routine Diagnostic Laboratory
Author(s) -
Janet C. Patton,
Eveline Akkers,
Ashraf Coovadia,
Tammy Meyers,
Wendy Stevens,
Gayle Sherman
Publication year - 2007
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00223-06
Subject(s) - heel , human immunodeficiency virus (hiv) , virology , venous blood , nucleic acid , dried blood , medicine , spots , virus , dried blood spot , biology , pathology , chromatography , chemistry , biochemistry , anatomy
The diagnostic accuracy of the Roche Amplicor human immunodeficiency virus type 1 DNA PCR assay (version 1.5) on DNA extracted from pediatric heel prick dried blood spots using Roche MagNA Pure nucleic acid purification technology was evaluated. The methodologies transfer successfully from the labor-intensive research laboratory to the high-throughput automated routine laboratory.