Open AccessInfluence of the Tissue Microenvironment on Toll-Like Receptor Expression by CD11c+Antigen-Presenting Cells Isolated from Mucosal Tissues
Author(s) -
Shunsuke Takenaka,
Sarah McCormick,
Ekaterina Safroneeva,
Zhou Xing,
Jack Gauldie
Publication year - 2009
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00216-09
Subject(s) - cd11c , microbiology and biotechnology , biology , immunology , receptor , antigen presenting cell , antigen , spleen , toll like receptor , immune system , t cell , innate immune system , phenotype , biochemistry , gene
It is recognized that functional activities of antigen-presenting cells (APCs) in mucosal tissue sites differ from those of systemic APCs; however, it is unknown whether there are further differences between APC populations residing in different mucosal sites. In this study, we directly compared murine CD11c+ APCs isolated from colon, lung, and spleen and found that APCs isolated from these tissues differ considerably in Toll-like receptor (TLR) expression and responses to in vitro TLR ligand stimulation. We also provide evidence that tissue microenvironments dictate distinct patterns of TLR expression by CD11c+ APCs in different mucosal tissues. Moreover, CD11c+ cells isolated from different tissues have varied capacities to induce the development of T helper 1 (Th1), Th2, or regulatory CD4+ T cells. Thus, unique tissue microenvironments have a significant influence on determining TLR expression by CD11c+ cells that migrate to and reside in each mucosal tissue and are likely to modulate their functional activities.