Open AccessMycobacterium tuberculosis Malate Synthase- and MPT51-Based Serodiagnostic Assay as an Adjunct to Rapid Identification of Pulmonary Tuberculosis
Author(s) -
Jacqueline M. Achkar,
Yuxin Dong,
Robert S. Holzman,
John T. Belisle,
Irene Kourbeti,
Tsering Sherpa,
Rany Condos,
William N. Rom,
Suman Laal
Publication year - 2006
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00158-06
Subject(s) - malate synthase , adjunct , tuberculosis , mycobacterium tuberculosis , pulmonary tuberculosis , microbiology and biotechnology , medicine , virology , immunology , biology , enzyme , pathology , biochemistry , linguistics , philosophy , glyoxylate cycle , isocitrate lyase
The 81-kDa malate synthase (MS; Rv 1837c) and the 27-kDa MPT51 (Rv 3803c) ofMycobacterium tuberculosis are immunodominant antigens recognized by serum antibodies from ∼80% of human immunodeficiency virus-negative smear-positive tuberculosis patients from India. We now provide evidence that the use of the MS/MPT51-based serodiagnostic assay can serve as an adjunct to sputum microscopy in the rapid diagnosis of pulmonary tuberculosis.